Ali M. A. Hassan, Atef F. El Hadad, Abd El Shakour A.H.
El-Mohammady, Olfat G. Shaker, Magdy E.H. Attaby*
Faculty of Science.- Al-Azhar University -Cairo
Background: semen is an organic fluid also known as seminal fluid that usually contains spermatozoa. It is secreted by the gonads (sexual glands) and other sexual organs of male and can fertilize female ova. In human, seminal fluid contains several components besides spermatozoa. Photolytic and other enzymes as well as fructose are elements of seminal fluid which promote the survival of spermatozoa and provide a medium through which they can move or swim. The process that results in the discharge of semen is called ejaculation. Male infertility is defined by most authorities as the inability to achieve a pregnancy for at least one year. Objectives: The study used resazurin reduction test as additional, inexpensive, simple tool for diagnosis of male infertility . Methods: This study was carried on patients obtained from outpatient clinic of male infertility department at El Hussein Hospital of Cairo. The mean age of investigated patients was 27±7 years. Samples were collected by masturbation in wide sterile container after 3 days of abstinence. Samples were divided into two groups. Group one comprised (n=30) normal controls (normozoospermia) indicated by sperm count more than 20 millions/ml, motility more than 50% and normal morphology. Infertile group consisted of oligozoospermia (n=30) indicated by sperm count less than 20 millions/ml, athenozoospermia (n=30) indicated by motility less than 50% and azoospermia (n=30) indicated by the complete absence of spermatozoa. All samples were centrifuged at 4000 rpm for 15 minutes and seminal plasma was separated and kept until analyses. Seminal plasma was collected on sterile dry test tube to separate for the biochemical studies (semen quality concentration) by using resazurin reduction
test (RRT). Results revealed that a highly significant decrease in semen quality was observed in male infertile (oligozoospermia, athenozoospermia and azoospermia) groups compared with findings of the control group. And significant decrease in semen quality was observed in azoospermia group and athenozoospermia group compared with findings of oligozoospermia group. Conclusion the semen quality concentration can be used as a new tool in the differentiation between the male infertile patients and normal cases, moreover it helps in differentiation between oligozoospermia, athenozoospermia and azoospermia.
Keywords: Seminal fluid, semen quality, male infertility, resazurin reduction test.