Estimation of Zinc in Seminal Fluid among Infertile Men
Ali M. A. Hassan, Atef F. El Hadad, Abd El Shakour A.H.
El-Mohammady, Olfat G. Shaker, Magdy E.H. Attaby*
Faculty of Science.- Al-Azhar University -Cairo
The objective of the present study is estimation of zinc (Zn) concentration in seminal fluid among infertile men. Methods: Cross section study was done to estimate different Zn concentrations in seminal fluid male patients recruited from outpatient clinic of male infertility department at El Hussein Hospital of Cairo (fertile and infertile males). Seminal fluid samples were collected by masturbation after 3 days of abstinence. Shortly after collection, semen samples were analyzed for liquefaction times, volume, leukocyte count, motility, morphology and count of spermatozoa. Seminal samples were divided into four groups according to the sperm counts; 30 normozoospermia, 30 oligozoospermia, 30 azoospermia and 30 athenozoospermia. The Zn concentrations were estimated in the four collected seminal groups using colorimetric method. The results revealed a highly significant decrease in Zn concentration in infertile male samples (oligozoospermia, athenozoospermia and azoospermia) compared to the normozoospermia samples. Furthermore a significant decrease in Zinc concentration was observed in azoospermia group compared to athenozoospermia and oligozoospermia samples.
Conclusion: Low Zn concentrations were detected in the seminal fluids of the infertility patients compared to the seminal fluid of the fertile males.
Keywords: Seminal fluid, Zinc, male infertility, colorimetric method.