Nanis G. Allam1*, EhabMostafa M. Ali2, SamyaShabanna1 and Elsayed Abd-Elrahman1
1Microbiology unit, Botany Department, 2Biochemistry unit, Chemistry Department,
Faculty of Science, Tanta University, Egypt.
Heavy metals contamination in Food and water were increased due to the environmental
pollution. Cadmium (Cd) and Lead (Pb) are regarded as the most toxic heavy metals,
wide occupational and environmental pollutants, found in soil, water and air. Probiotics
especially lactic acid bacteria (LAB) have been found to bind and remove, have the
capacity to decrease tissue heavy metals levels and heavy metals-induced oxidative stress.
The objective was to screen number of different LAB strains for their potential probiotic
capacity against heavy metals toxicity. LAB tolerance against Cd and Pb were
investigated first and the most tolerant strain for Cd was selected for cadmium binding
capacity. Antioxidative properties for all tested stains were investigated through
determination of α,α-Diphenyl-β-Picrylhydrazyl (DPPH) and inhibition of lipid
peroxidation in rat liver homogenate in vitro. The results revealed that LAB strains
showed high tolerance against Cd and Pb and have antioxidative activities. S.thermophilus
was the most tolerant strain against Cd, removed 70% of Cd after 1 hour from solution
and also revealed good antioxidative properties. These results suggest that S. thermophilus
and LAB strains could be used as efficacious nutritional dietary supplement in the daily
life to prevent and reduce heavy metal toxic effect.