Prevalence of Legionella spp. and Helicobacter pylori in different water resources in Egypt
Mohamed Azab El-Liethya*, Bahaa A. Hemdana, Einas H. El-Shatouryb, Mohamed A. Abou-Zeidb, Farag A. Samhana, Gamila E. El-Taweela
aBacteriology Lab., Water Pollution Research Department, Environmental Research Division, National Research Centre, Dokki, Giza,12622, Egypt. bMicrobiology Department, Faculty of Science, Ain Shams University. El- Khalifa El-Mamon St., Abbassia, Cairo, 11566, Egypt
The main objective of this investigation was to detect Legionella spp. and Helicobacter pylori in different types of water resources. One hundred seventy five different water samples were collected from Egypt. Water samples were collected from Nile River (Rossita Branch), the Mediterranean Sea at Marsa Matroh shore, El-Rahawy Drain, hospital wastewater and groundwater. Legionella spp. and Helicobacter pylori were determined using membrane filtration and spread plate techniques. Legionella spp. and H. pylori were detected using culture methods on selective media in 25 and 33% of the total examined water samples, respectively. The biochemical tests for Legionella spp. showed that, 6 (35%) out of 17 might belong to L. pneumophila and 4 (23%) out of 17 belonged to non-pneumophila Legionella species. Eight out 27 (29%) isolates showed positive results for urease, catalase, oxidase, motility tests and negative nitrate reduction were confirmed as H. pylori. The results concluded that further monitoring and identification should be carried out in the future for the presence pathogens in water resources.