Thursday, 16 February 2017 03:42

Identification of RAPD and ISSR Markers for Drought Stress in Some Egyptian Durum Varieties

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Identification of RAPD and ISSR Markers for Drought Stress in Some Egyptian Durum Varieties

1Haiba, A.A.A., 2M.A.H.Youssef, 1S.A.A. Heiba*, 1HodaB. M. Ali and 2A. S. Ibrahim

1Genetics and Cytology Department, Division of Genetic Engineering and Biotechnology, National Research Centre (NRC), Cairo, Egypt. 2Genetics Department, Faculty of Agric., Zagazig University, Egypt. *Corresponding author: S.A.A., HeibaE. mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Abstract

To develop crop plants with enhanced tolerance of drought stress, a basic understand of physiology and genetics is essential. Six durum wheat genotypes namely Bani-Swaif 1, 3, 4, 5, 6 and Sohag3 were first screened to get the most tolerant and sensitive genotypes. Then the tolerant, sensitive plants and their F1 and F2 were used for molecular indicators of drought tolerance using RAPD and ISSR techniques. Six RAPD and five ISSR primers were used to identify markers assisted selection (MAS) of drought tolerance. Under this study, RAPD technique exhibited 4 positive and 5 negative markers while ISSR revealed 6 positive and 6 negative markers.

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