Monday, 10 April 2017 13:02

Morphological and Molecular Detection of Potentially Pathogenic Free-Living Amoebae in Swimming Pool Samples

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Amoebae in Swimming Pool Samples
Ahmad Z. Al-Herrawy*1; Mahmoud A. Gad1; Ahmed Abd El-Aziz2; Mouhamed F. Abou-El Nour2; Fayz M. Shaldoum2;Amgad Salahuldeen2
1- Environmental Parasitology Lab., Water Pollution Research Dept., National Research Centre, 12622 Dokki, Giza, Egypt.
2- Zoology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt
* Corresponding author: E.mail: This email address is being protected from spambots. You need JavaScript enabled to view it. phone: +201001838830
Abstract
Free-living amoebae (FLAs) include several medically important genera such as Naegleria spp. Acanthamoeba spp. and Hartmannella spp. A total of 48 water samples were collected from swimming pool samples (24 water and 24 swab samples). Samples were processed for detection of free-living amoebae using non-nutrient agar (NNA). The isolates of free-living amoebae were microscopically identified to the genus level based on the morphologic criteria and molecularly confirmed by the aid of PCR using genus-specific primers.
The results of the present study showed that the highest percentage of FLAs was recorded in swimming pool swab samples (70.8), while the lowest was recorded in swimming pool water samples (54.2%). Seasonally, the highest occurrence percentage of FLAs in swimming pool water samples was observed in summer season (100%) then it decreased to be 66.7, 33.3, and 16.7% in spring, autumn and winter, respectively. Genus Acanthamoeba was the most prevalent genus in swimming pool swab samples (54.2%) as well as swimming pool water samples (33.3%). Morphological identification of the isolated FLAs showed the presence of 3 genera namely Acanthamoeba, Naegleria and Vermamoeba (Hartmannella) that were confirmed by PCR.
In conclusion, the appearance of FLAs especially potentially pathogenic amoebae in swimming pool samples may cause a potential health threat.

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