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Sandwich ELISA & Conventional PCR for Diagnosis of Schistosoma mansoni infection (A Field Study)

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Sandwich ELISA & Conventional PCR for Diagnosis of Schistosoma mansoni infection (A Field Study)
Eman M.H. Méabed1, Eman E.Taher2*, Nancy O. Kamel3, Dina M. H. El Akkad4 and M.M. El-Bahy5
Parasitology Department, Faculty of Medicine, Fayoum University1
Clinical Parasitology Unit, Research Institute of Ophthalmology2
Parasitology Department, Faculty of Medicine, October 6 University3
Parasitology Department, Faculty of Medicine Cairo University4
Parasitology Department, Faculty of Veterinary Medicine, Cairo University5
*Corresponding author: Eman E.Shabrawi Taher, Clinical Parasitology Department, Research Institute of Ophthalmology, Giza, Egypt .
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Abstract
Schistosomiasis is a major public health problem. Accurate early diagnosis is strongly recommended to provide proper treatment before progressing to complications. Objective and methods: the aim of this study was to detect S. mansoni infected cases in two villages at Fayoum Governorate, Egypt, through application of different diagnostic methods. In field, subjects were primary screened by Kato Katz (KK) and Sandwich Enzyme Linked Immunosorbent Assays (ELISA). ELISA was performed for antigen detection in both serum and saliva samples. Then conventional Polymerase Chain Reaction (PCR) was used to examine 366 selected preserved serum samples, and results were compared with earlier tests.
Results: Among 1330 subjects completed the study and gave the required samples, KK revealed prevalence of S. mansoni infection among the study population (1.8%). In comparison with the standard KK technique, ELISA detecting antigen in serum and/or saliva samples gave sensitivity rates up to 100% and specificity rates (≥ 99%). PCR sensitivity and specificity were (95.8% & 96.2%) in comparison with KK.
Conclusion: S. mansoni is still prevalent in the study areas with low rate of transmission. Sandwich ELISA assay for S. mansoni circulating antigen detection is a sensitive and specific
Egyptian Journal of Environmental Research EJER, Vol. 5, October 2016
27
diagnostic tool (either applied on serum or saliva samples) with results in the same range with PCR.

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