Nanis

Nanis

Evaluation of Microbial Quality of the River Nile Water at El-Gharbia Governorate, Egypt in Response to Seasonal and Regional Variations

Nanis G. Allam*,Walaa Mustafa El-Gemaizy
Microbiology Unit, Botany Department, Faculty of Science, Tanta University, Tanta, Egypt.

Abstract
Microbial (bacterial, algal and fungal) quality of the River Nile water at El-Gharbia governorate
was elucidated during December 2012 - November 2013. Water samples were collected from
mostly polluted sites of twenty four sites at the River Nile of El-Gharbia governorate. Detection
of bacterial indicator count involved total coliform (TC), fecal coliform (FC) and Escherichia
coli (E.coli) revealed increase in summer. Also, some pathogenic bacteria had been detected and
the most frequent were Staphylococcus aureus and E.coli. Algal count was ranging between 54
to 3933 (organisms/ L) and the mostly present algal species were Cyanophyta (Oscillatoria
anguiformis, Nostoc muscorum and Anabaenaconstricta),Chlorophyta (Scenedesmus
brasiliensis and Chlorella vulgaris) and Bacillariophta (Diatoms). Assessment of fungal
contamination in investigated water samples revealed the presence of Aspergillus spp,
Penicillium spp and Fusarium spp and yeasts especially Candida sp during four seasons
especially in summer.

Read more...

The role of probiotic bacteria in removal of heavy metals

Nanis G. Allam1*, EhabMostafa M. Ali2, SamyaShabanna1 and Elsayed Abd-Elrahman1
1Microbiology unit, Botany Department, 2Biochemistry unit, Chemistry Department,
Faculty of Science, Tanta University, Egypt.

Abstract
Heavy metals contamination in Food and water were increased due to the environmental
pollution. Cadmium (Cd) and Lead (Pb) are regarded as the most toxic heavy metals,
wide occupational and environmental pollutants, found in soil, water and air. Probiotics
especially lactic acid bacteria (LAB) have been found to bind and remove, have the
capacity to decrease tissue heavy metals levels and heavy metals-induced oxidative stress.
The objective was to screen number of different LAB strains for their potential probiotic
capacity against heavy metals toxicity. LAB tolerance against Cd and Pb were
investigated first and the most tolerant strain for Cd was selected for cadmium binding
capacity. Antioxidative properties for all tested stains were investigated through
determination of α,α-Diphenyl-β-Picrylhydrazyl (DPPH) and inhibition of lipid
peroxidation in rat liver homogenate in vitro. The results revealed that LAB strains
showed high tolerance against Cd and Pb and have antioxidative activities. S.thermophilus
was the most tolerant strain against Cd, removed 70% of Cd after 1 hour from solution
and also revealed good antioxidative properties. These results suggest that S. thermophilus
and LAB strains could be used as efficacious nutritional dietary supplement in the daily
life to prevent and reduce heavy metal toxic effect.

Read more...